Zesttee's 6 Step Blood Spot Collection Manual

How to Collect Blood Spot Samples

Make sure to register your kit at zesttee.com/kit before you proceed.

Step 1

Make sure to complete the test requisition form and sign it. Without your signature, we cannot perform the testing.

Step 2

Open your collection card on a flat surface, and write your name (make sure it matches your registration!) and the collection date. This informs the lab of whose sample they are processing.

Step 3

Choose your ring or pinkie finger and use the alcohol pad to wipe the fingertip clean. The recommended puncture site is slightly off the middle of the fingertip.

Step 4

Then, twist and pull the safety end off the lancet. Because the lancet will only activate once, make sure to keep your finger on a hard surface. To puncture, press the lancet down until you hear a click.

Step 5

Wipe the first drop away and then apply downwards pressure from the base of your finger toward the puncture site. Fill each circle on the collection card with one drop of blood until all 5 circles are filled. Once finished, cover your fingertip with a bandaid and keep the card open to allow it to dry completely.

Step 6

Place your sample into the biohazard bag and then into the green smartpac, and seal it. Make sure to write your address. Drop it off at any post box.

Properly Collected Specimen

Collection Tips

  • Drink plenty of water prior to collecting your sample to ensure that you're hydrated.
  • Ensure your hands are warm by thoroughly rinsing your hands in warm water or showering. If you have calluses on your fingers, we suggest soaking your hands in warm water to soften the skin before collection.
  • Promote adequate blood flow by swinging your arms by your side or by exercising beforehand.
  • Many find it helpful to have someone assist them in the collection process.
  • Once your finger is punctured, wipe away the first drop of blood.
  • If you find that a drop is not forming, it's helpful to promote blood flow by massaging from your wrist down to your fingertip.
  • The additional lancets can be used if needed but it's recommended to choose a different finger.

What Not To Do

  • Do not rub or smear (allow full drops to form and gently fall onto the paper).
  • Do not allow multiple drops in the same circle (no overlapping).
  • Do not use heat to assist drying.
  • Please Note: Under no circumstances should you use materials other than the lancets included in your test kit.

    If you require assistance with your collection, please contact our team by emailing [email protected].

Complete Paperwork

Write your name, collection date, and time in the space provided on the collection card. Complete the test requisition form and sign it. Without your signature we cannot perform the testing.


Place your sample back into the smartpac and drop it off at any post box for it to be shipped back to us.

If you require assistance with your collection, please contact our team by emailing [email protected].